Serum protein electrophoresis interpretation pdf

Labcorp test details for protein electrophoresis with interpretation, serum with reflex to ife, serum, and free. Common problems associated with the interpretation of serum protein electrophoresis are summarized in box 7. Onedimensional protein electrophoresis separates the proteins in serum or urine into their main classes albu min and alpha, beta and gamma globulins on. Two major types of proteins can be identified with. Serum protein electrophoresis and its clinical applications. The serum protein electrophoresis procedure is intended for the separation and quantitation of serum proteins using cellulose acetate electrophoresis. Summary serum contains over one hundred individual proteins, each with a specific set of functions and subject to specific variation in concentration under different pathologic conditions. The most important aspect of such albumin variants lies in quantification of an albumin peak in such scenarios followed by interpretation and clinical correlation. Understanding and interpreting the serum protein electrophoresis. Pdf serum protein electrophoresis is used in clinical practice to identify.

The subsets of these proteins and their relative quantity are the primary focus of the interpretation of serum protein electrophoresis. A hemoglobin electrophoresis test is a blood test used to measure and identify the different types of hemoglobin in your bloodstream. Serum protein electrophoresis is a laboratory examination that commonly is used to identify patients with multiple myeloma and other disorders of serum protein. Understanding and interpreting serum protein electrophoresis. Serum protein electrophoresis is used to identify patients with. Serum protein electrophoresis spep is a laboratory technique thats used to determine the level of some types of proteins in a blood sample. Dubois lupus erythematosus and related syndromes eigth edition, 20. Abnormal serum protein electrophoresis pattern in a. Hemoglobin is the protein inside red blood cells responsible. The total serum protein test measures all the proteins in your blood. In protein electrophoresis, two major groups of proteins can be distinguished. The gammaglobulin zone is decreased in hypogammaglobulinemia and agammaglobulinemia.

Electrophoresis separates proteins based on their physical properties, and the subsets of these proteins are used in interpreting the results. An immunofixation tutorial presented by john okeefe sha robinson, phd rita. In some cases, additional time should be allowed for additional. Serum protein electrophoresis an overview sciencedirect topics. Serum protein electrophoresis spep is a widely available and inexpensive laboratory test that examines specific serum proteins based on their physical properties. Protein electrophoresis with interpretation, serum. In other cases, patients may have nonspecific symptoms, meaning the discovery is. The subsets of these proteins and their relative quantity are serum protein electrophoresis is used to identify patients with multiple myeloma and other serum protein disorders. Serum protein electrophoresis spep is an easy, inexpensive method of separating proteins based on their net charge, size, and shape. Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. Making sense of serum protein bands serum protein bands monoclonal gammopathy will sometimes be found following serum protein electrophoresis in patients presenting with classic signs or symptoms of multiple myeloma, e. Understanding and interpreting serum protein electrophoresis csv. It can also check the amount of albumin you have compared to globulin, or whats called your ag ratio.